CPSF3 Promotes Pre-mRNA Splicing and Prevents CircRNA Cyclization in Hepatocellular Carcinoma

CircRNAs are crucial in tumorigenesis and metastasis, comprehensively downregulated hepatocellular carcinoma (HCC). Previous studies demonstrated that the back-splicing of circRNAs was closely related to 3′-end splicing. As a core executor cleavage, we hypothesized CPSF3 modulated circRNA circularization. Clinical data were analyzed establish prognostic correlations. Cytological experiments performed determine role HCC. A fluorescent reporter employed explore mechanism. The regulated by screened RNA-seq validated PCR, changes downstream pathways explored molecular experiments. Finally, safety efficacy inhibitor JTE-607 verified both vitro vivo. results showed highly expressed HCC cells, promoting their proliferation migration, high level predictive poor prognosis. mechanistic study revealed enhanced RNA thereby reducing circRNAs, increasing linear mRNAs. Furthermore, inhibition JET-607 suppressed cells. Our findings indicate increase promotes shift pre-mRNA from mRNA, leading uncontrolled cell proliferation. exerted therapeutic effect on blocking CPSF3.